polyjet in vitro dna transfection reagent (SignaGen)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

SignaGen polyjet in vitro dna transfection reagent
Polyjet In Vitro Dna Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+in+vitro+dna+transfection+reagent/polyjet+dna+transfection+reagent/pmc09481576__41467_2022_33111_MOESM1_ESM-205-22-28
Average 90 stars, based on 1 article reviews

polyjet in vitro dna transfection reagent - by Bioz Stars, 2026-09

90/100 stars

Images

Western Blot:

Article Title: CRAMP1 drives linker histone expression to enable Polycomb repression.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit ɑ-CRAMP1 (used for immunoblot) Atlas Antibodies Cat#HPA041752; RRID:AB_10796459 rabbit ɑ-CRAMP1(used for IP and CUT&RUN) Fortis Life Sciences Cat#A304-094A; RRID:AB_2621343 mouse ɑ-ɑ-Tubulin Cell Signaling Technologies Cat#3873; RRID:AB_1904178 rabbit ɑ-GAPDH Cell Signaling Technologies Cat#2118; RRID:AB_561053 rabbit ɑ-HP1ɑ Cell Signaling Technologies Cat#2616; RRID:AB_2070987 rabbit ɑ-H1.0 Proteintech Cat#17510-1-AP; RRID:AB_10695628 rabbit ɑ-H1.1 Abcam Cat#ab254394; RRID:AB_3677544 rabbit ɑ-H1.2 Proteintech Cat#19649-1-AP; RRID:AB_10694432 rabbit ɑ-H1.3 BioRad Cat#VPA00783; RRID:AB_3677545 rabbit ɑ-H1.4 Cell Signaling Technologies Cat#41328; RRID:AB_2799199 rabbit ɑ-H1.5 Abcam Cat#ab18208; RRID:AB_470263 rabbit ɑ-H1X Fortis Life Sciences Cat#A304-604A; RRID:AB_2620799 mouse ɑ-β-actin Merck Cat#A2228; RRID:AB_476697 rabbit ɑ-H2A Active Motif Cat#39235; RRID:AB_2687477 mouse ɑ-H2B Abcam Cat#52484; RRID:AB_1139809 rabbit ɑ-H3 Cell Signaling Technologies Cat#4499; RRID:AB_10544537 mouse ɑ-H4 Abcam Cat#ab17036; RRID:AB_1209245 rabbit ɑ-SUZ12 Proteintech Cat#20366-1-AP; RRID:AB_10694152 mouse ɑ-FLAG Merck Cat#F1804; RRID:AB_262044 rabbit ɑ-H3K27me3 (used for immunoblot) Merck Millipore Cat#07-449; RRID:AB_310624 rabbit ɑ-H3K27me3 (used for CUT&RUN and CUT&Tag) Epicypher Cat#13-0055; RRID:AB_3665059 mouse ɑ-V5 Abcam Cat#ab27671, RRID:AB_471093 rabbit ɑ-IgG EpiCypher Cat#13-0042; RRID:AB_2923178 rabbit ɑ-H3K9me3 Abcam Cat#ab8898; RRID:AB_306848 ɑ-HIS-24 (raised against amino acids 37-113) This manuscript (Produced by Strategic Diagnostics International) N/A rabbit ɑ-NPAT Fortis Life Sciences Cat#A302-772A; RRID:AB_10630262 rabbit ɑ-GON4L Atlas Antibodies Cat# HPA057305; RRID:AB_2683408 Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Cat#711-035-152; RRID:AB_10015282 Peroxidase AffiniPure Mouse Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Cat#715-035-150; RRID:AB_2340770 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat#C2987 Chemicals, peptides, and recombinant proteins PolyJet in vitro DNA transfection reagent SignaGen Laboratories Cat#SL100688 Hygromycin B Thermo Fisher Scientific Cat#10687010 Blasticidin S HCl Stratech Cat#B4879 Puromycin Dihydrochloride Thermo Fisher Scientific Cat#A1113803 T4 Polynucleotide Kinase New England Biolabs Cat#M0201L T4 DNA Ligase New England Biolabs Cat#M0202L DNase I Qiagen Cat#79256 Pierce Protein A Magnetic Beads Thermo Fisher Scientific Cat#88846 Pierce Protein G Magnetic Beads Thermo Fisher Scientific Cat#88848 (Continued on next page) Molecular Cell 85, 1–14.e1–e8, July 3, 2025 e1 .. hTERT RPE-1 ATCC #CRL-4000 HEK-293T Gift from Prof. Paul Lehner N/A KBM-7 Gift from Prof. Paul Lehner N/A Jurkat Gift from Prof. Paul Lehner N/A HCT116 Gift from Prof. Steve Jackson N/A Oligonucleotides See Table S11 This manuscript N/A Recombinant DNA pHRSIN-pSFFV-V5-CRAMP1-IRESmCherry-WPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-V5-CRAMP1dSANTIRES-mCherry-WPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-V5-CRAMP1dDomIIIRES-mCherry-WPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-V5-CRAMP1dUBL-IRESmCherry-WPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-FLAG-H1.2-IRES-mCherryWPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-FLAG-H1.3-IRES-mCherryWPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-FLAG-H1.4-IRES-mCherryWPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-FLAG-H1.5-IRES-mCherryWPRE-pPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.1-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.2-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.3-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.4-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.5-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A pHRSIN-pSFFV-H1.0-IRES-mCherry-WPREpPGK-Hygromycin R This manuscript N/A (Continued on next page) Molecular Cell 85, 1–14.e1–e8, July 3, 2025 e3

Produced:

Virus:

Recombinant:

In Vitro:

Article Title: Unleashing the Potential of Noncanonical Amino Acid Biosynthesis to Create Cells with Precision Tyrosine Sulfation
Article Snippet: .. To confirm the genetic incorporation of sTyr from either biosynthesis or external addition, HEK293T and HEK293T-NnSULT1C1 cells were transfected with pAcBac2.tR4-sTyrRS/GFP* with Polyjet In Vitro DNA Transfection Reagent (SignaGen Laboratories) in the presence or absence of 3 mM sTyr addition. ..

Article Title: CRAMP1 drives linker histone expression to enable Polycomb repression.
Article Snippet: .. HEK-293T cells were transfected with the target lentiviral transfer plasmid plus four packaging plasmids encoding Gag-Pol, Rev, Tat and VSV-G respectively using PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories, #SL100688). .. Untransduced KBM-7 cells were eliminated using either 500 μg/ml hygromycin (ThermoFisher Scientific, #10687010), 6.7 μg/ml blasticidin (Stratech, #B4879) or 0.75 μg/ml puromycin (ThermoFisher Scientific, #A1113803); untransduced K562 cells were eliminated using either 250 μg/ml hygromycin, 10 μg/ml blasticidin or 1 μg/ml puromycin.

Article Title: Heat-Induced Phosphatidylserine Changes Drive HSPA1A's Plasma Membrane Localization.
Article Snippet: .. After 18 hours, cells were transfected with the appropriate construct using the PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories, Frederick, MD, USA), following the manufacturer's instructions. ..

Article Title: Mechanisms of USP18 specificity toward ISG15 revealed by paralog sequence analysis comparison
Article Snippet: HEK-293T cells were obtained from ATCC and grown in DMEM complete medium (Gibco, Cat# 11–965–092) supplemented with 10% fetal bovine serum (Atlanta Biologicals). .. All DNA transfection experiments were done using PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories) according to the manufacturer's instructions and subsequently cultured for 24 to 48 h prior to analysis. ..

Article Title: Gene therapy for ocular hypertension using hfCas13d-mediated mRNA targeting
Article Snippet: The cells were seeded onto 12-well poly- D- lysine-coated plates (Corning Life Sciences). .. Approximately 12–15 h after plating, cells were transfected with 2.25 μL of PolyJet In Vitro DNA Transfection Reagent (SignaGen, Catalog #: SL100688) using 375 ng of hfCas13d or hfCas13x plasmids and 375 ng of gRNA plasmids. .. As mentioned in our previous study , all in vivo experiments were conducted in accordance with the guidelines set by the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the Institutional Animal Care and Use Committee at Stanford University School of Medicine.

Article Title: Precise Correction of the Pde6b -L659P Mutation Causing Retinal Degeneration with Minimum Bystander Editing by Advanced Genome Editing Tools
Article Snippet: The N2a cell line was maintained in Dulbecco’s Modified Eagle’s Medium (Corning, #10013CV) supplemented with 10% fetal bovine serum (FBS) under standard conditions of 37°C with 5% CO2. .. For transfection, cells were plated in 24-well plates and treated with PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories, #SL100688). .. Specifically, 1.5 μl of PolyJet reagent was mixed with a total of 500 ng of BE, PE, or CE plasmids per well.

Article Title: SARS-CoV-2 ORF7a activates endothelium to release von Willebrand factor that promotes thrombosis
Article Snippet: A murine endothelial cell line (SVEC4-10) (Gaithersburg, MD) and HEK 293 cells that were transiently or stably transfected were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning cellgro, Glandale, AZ) with 10% FBS and 50 IU/mL penicillin and streptomycin, maintained at 37°C in a 5% CO2 humidified incubator. .. Transfection and expression of Orf7 protein: At 70-80% confluence, HEK 293 cells or SVECs were transfected with pCMV6-Orf7a-mCh-Flag or an empty control vector using PolyJet In Vitro DNA Transfection Reagent (SignaGen Labs, Houston, TX) following the manufacturer’s instructions. ..

Review

polyjet in vitro dna transfection reagent (SignaGen)

Structured Review

Images

Related Articles

Western Blot:

Produced:

Virus:

Recombinant:

In Vitro:

Transfection:

Magnetic Beads:

Plasmid Preparation:

Construct:

Cell Culture:

Expressing:

Control:

Similar Products

Image Search Results